As we discussed before, fatty tissue is fundamentally tough to penetrate, so when picking out your agent bit of tissue, you have got to Understand that some trimming will probably be demanded. You need to get the specimen down to a great dimensions. Optimally We have now 1 to two-millimeter biopsies for quick processing , and We've three to four mi
This is often especially legitimate for immunohistochemistry by which 10% NBF continues to be proven to generally be a lousy fixative (Fig. two). Some problems with the use of ten% NBF as a fixative are actually corrected through the introduction of other experimental tactics including antigen retrieval (Shi et al. 2001) and using tiny amplicons in
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